Reporter

Part:BBa_K1865001:Design

Designed by: Avril von Hoyningen-Huene, Sindhu Thiagarajan, Verena Siebert   Group: iGEM15_Goettingen   (2015-09-17)


eforRed_BCEL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Composite BioBrick was assembled with alternative restriction sites into our expression vector pBAD. In this BioBrick the two sequences can be separated into their composite parts by using KpnI to cut in between the two. The outer restriction sites are EcoRI (prefix) and PstI (Suffix).


Source

BCEL was isolated from the DNA of Bacteroides cellulolyticus. eforRed was used from a gBlock with the BioBrick sequence of BBa_K592012.

References